Abstract
The taxonomy research on Antarctic macroalgae is important for the protection of the Antarctic environment and ecology and some economic exploitation of the Antarctic algae. At present, the classification and identification of Antarctic macroalgae still focus on morphological methods, however, this lacks DNA barcoding using molecular methods. This study identified 114 samples of macroalgae from the Great Wall Station, using morphological and DNA barcoding identification. The results show that pure DNA of samples was difficult to obtain, which affected the efficiency of amplification and sequencing. Among them, the sequence obtained rate of COI gene was only 29% in red algae. The ITS sequence of brown algae only aligned to 8 reference sequences, belonging to 2 algal orders. The lack of reference sequences of Antarctic macroalgae increases the difficulty of DNA barcoding identification. With morphological and molecular identification of the samples in this study, 114 samples of Antarctic macroalgae were identified and classified as 21 genera s, 16 families, 14 orders, and 3 phyla. These species are Chaetomorpha tortuosa, Monostroma angicava, Ulothrix flacca, Ulva sp., Protomonostroma sp., Desmarestia Antarctica, Desmarestia confervoides, Himantothallus grandifolius, Haplogloia andersonii, Colpomenia bullosa, Petalonia fascia, Saccharina dentigera, Cystosphaera jacquinotii, Ascoseira mirabilis, Porphyra endiviifolia, Iridaea cordata, Pachymenia orbicularis, Palmaria decipiens, Georgiella confluens, Hymenocladiopsis prolifera, Ploca mium cartilagineum, and belong to 21 genera s, 16 families, 14 orders, and 3 phylums. This study systematically identifies the Antarctic macroalgae using DNA barcoding, providing basic molecular information about Antarctic macroalgae.
Presenters
Sohaib KhanPhD Student, Department of Experimental Medicine, University of Rome, Tor Vergata, Agrigento, Italy
Details
Presentation Type
Paper Presentation in a Themed Session
Theme
KEYWORDS
ECOLOGICAL DISTRIBUTION, MACROALGAL COMMUNITIES OF ANTARCTICA, DNA BARCODING, BIODIVERSITY
