Abstract
Prebiotics are ingredients selectively fermented by the intestinal microbiota that promote changes in its structure and/or metabolism, conferring health benefits to the host. This research focused on prebiotics production, specifically β (1-4) GOS, and their potential role in colorectal cancer (CRC). We present studies on a potential role in CRC progression to finally describe the current state of β (1-4) GOS generation for industrial production. Research was carried out in two phases, production of prebiotic galacto-oligosaccharide and assessment of their protective role against colon cancer in Male Sprague Dawley rats. Results: Greatest percentage of enzyme activity was observed at 6.5 pH and 37oC. Transgalactosylation process was carried out at 37°C by the use of enzymes with three different concentrations. The animals body mass belonging to galacto-oligosaccharides (Group G2, G3 and G4) increased suggestively higher as associated to control group (G1). Effect of prebiotic treatment had significant effects on organ weights; liver, heart, spleen as well as small intestine length. Conclusively treated galacto- oligosaccharides groups and inulin group had significantly lowest aberrant crypt foci (ACF) counts per colon in contrast to controlled animals (G1 and G5). Present results were found to be defensive in contradiction of colon cancer. More research is needed at DNA level to explore the mutations at gene levels.
Presenters
Naureen NaeemAssociate Professor, Forensic Sciences, Lahore Garrison University, Punjab, Pakistan Bisma Naeem
Lecturer, Computer Science, Lahore Garrison University, Punjab, Pakistan
Details
Presentation Type
Paper Presentation in a Themed Session
Theme
KEYWORDS
PREBIOTIC, COLON CANCER, ABBERANT CRYPT FOCI, GALACTO-OLIGOSACCHARIDES
