Abstract
To understand the physiological and molecular mechanisms underlying the swimming behavior of fish under water velocity is crucial for insights into their adaptability and survival. The current study studied the difference in swimming performance under various water flow velocities and examined the associated gene regulation pattern. Grass carp were subjected to controlled water flow with water velocities to measure the critical swimming speed (Ucrit) which showed the water velocities changed swimming behavior and the swimming speed was significantly increased (p < 0.05) with body length. Additionally, brain samples were collected for RNA sequencing, which revealed that differentially expressed genes (DEGs) were functionally annotated revealing key pathways associated with changed behavior patterns. The Enrichment analysis resulted significant variation of rich factors in all groups including behavior (p < 0.05), skeletal system development (p < 0.05), hormone activity (p < 0.05), muscle contraction (p < 0.05**), locomotion (p < 0.05), and swim bladder development (p < 0.05) were found the major regulators of behavior in grass carp underwater velocities. Moreover, some genes were identified and found significantly different for enzymes and hormones, which could play a direct or indirect role during swimming behavior such as gene-ca7 (p < 0.005). The current study provides evidence of the neurogenetic mechanism underlying the changed swimming activity of grass carp underwater velocity, which could have important implications for understanding the impact of hydrodynamics and the fish.
Presenters
Mian AdnanPostdoc, College of Hydraulics and Environmental Engineering, China Three Gorges University, China
Details
Presentation Type
Paper Presentation in a Themed Session
Theme
Assessing Impacts in Diverse Ecosystems
KEYWORDS
Grass carp; Swimming behavior; Gene expression; Calcium signaling pathway; Hormonal
